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Image Search Results
Journal: Stem Cells Translational Medicine
Article Title: Chitosan Wound Dressings Incorporating Exosomes Derived from MicroRNA‐126‐Overexpressing Synovium Mesenchymal Stem Cells Provide Sustained Release of Exosomes and Heal Full‐Thickness Skin Defects in a Diabetic Rat Model
doi: 10.5966/sctm.2016-0275
Figure Lengend Snippet: Classification and modification of human SMSCs and exosomes. (A): SMSCs exhibited a spindle‐like morphology. Scale bar = 50 μm. (B): Flow cytometric analysis of cell surface markers. Blank curves represent isotype controls and solid gray curves represent test samples. (C): SMSCs displayed osteogenic, adipogenic, and chondrogenic differentiation potential. Scale bars = 50 μm (osteogenic), 20 μm (adipogenic), and 100 μm (chondrogenic). (D): mRNA levels detected by quantitative reverse‐transcriptase polymerase chain reaction. The experiment was repeated three times. ∗, p < .05 compared with unmodified SMSCs. (E): Particle size distribution of SMSC‐Exos measured by Nanosizer. The experiments were repeated three times, and representative results are shown. (F): Morphology of SMSC‐Exos analyzed by transmission electron microscopy. Scale bar = 200 nm. (G): Exosome surface markers detected by Western blotting (Alix, Tsg101, CD9, CD63, and CD81). Three independent experiments were performed to confirm the stability of these phenomena. Abbreviations: SMSC, synovium mesenchymal stem cell; SMSC‐126, microRNA‐126‐3p‐overexpressing synovium mesenchymal stem cells.
Article Snippet: Expression of the exosomal characteristic markers Alix (1:500),
Techniques: Modification, Reverse Transcription, Polymerase Chain Reaction, Transmission Assay, Electron Microscopy, Western Blot